Preparation and characterization of Neem Bark Extract with antimicrobial activity


  • Sudipta Roy Department of Pharmaceutics: Laboratory of Calcutta Institute of Pharmaceutical Technology and Allied Health Sciences, Banitabla, Uluberia, Howrah-711316, West Bengal, India
  • Devender Sharma Department of Pharmaceutics, Goenka College of Pharmacy, Sikar, Rajasthan, India


neem, antimicrobial, chopped bark


Aim and objective of this study was to prepare and characterize of Neem Bark Extract from Azadirachata indica (family – Meliaceae). In the mid of August, the stem bark was collected from the Azadirachata indica tree. After preparing neem bark methanolic extract from stem of Azadirachata Indica, extract was characterized by physical property evaluation, chemical property evaluation, antimicrobial evaluation, by determination of λ max using methanol and ethanol solvent, percentage of purity of prepared Azadirachta indica bark exracts using UV spectroscopic method(shimadzu-1700), doing structural elucidation by FTIR study in laboratory.


Gate pharmacy, Dr.Mohammed Ali, Eigth edition 2006-2007, Birla publications pvt. Ltd., 1-17.

Dr. Ravi Sankar United state patents, shimizu et al, patent No-4515785, date of patent-may 7, 1985.

Hossain, M.A., Al-Toubi, W.A.S., Weli, A.M., AlRiyami, Q.A., Al-Sabahi, J.N. 2013. Identification and characterization of chemical compounds in different crude extracts from leaves of Omani neem. J. Taibah. Univ. Sci. 7, 181–188.

Saleem S, Muhammad G, Bukhari Y NA and Hussain M A 2018 A comprehensive review of phytochemical profile, bioactives for pharmaceuticals and pharmacological attributes of Azadirachta indica Phytotherapy Research 1-32

Chen J, Fan X, Zhu J, Song L, Li Z, Lin F, Yu R, Xu H and Zi J 2018 Limonoids from seeds of Azadirachta indica A. Juss and their cytotoxic activity Acta Pharmaceutica Sinica B 8(4) 639-644 .

Gahlawat J, Sharma D, Thakur GS, Chobdar J, Sharma V.Formulation and evaluation of polyherbal liquid shampoo. European Journal of Biomedical and Pharmaceutical Sciences. EJBPS. 2019, 6(7):149-154.

Gupta S C, Prasad S, Tyagib A K, Kunnumakkarac A B and Aggarwal B B 2017 Neem an indian traditional panacea with modern molecular basis Phytomedicine 34 14-20.

Ghosh V, Sugumar S, Mukherjee A and Chandrasekaran N 2016 Neem (Azadirachta indica) oils Essential Oils in Food Preservation, Flavor and Safety 67 593-599.

Kumar V S and Navaratnam V 2013 Neem (Azadirachta indica) prehistory to contemporary medicinal uses to human kind Asian Pacific Journal Tropical Biomedicine 3(7) 505-514.







How to Cite

Preparation and characterization of Neem Bark Extract with antimicrobial activity. (2020). Journal of Public Health and Sanitation, 1(1), 7-16.